Melanocyte autologus stem cell transplantation non-invasive method (share with your Doctor)

Hi Friends,

I've been studying a lot how to do a non-invasive melanocyte stem cell transplantation (at home or at your family doctor clinic). It took me 4 months and 3 very thick books, to get to a step-by-step procedure, that has to be shared with a Doctor. Everything has to be sterilized.

The good thing is that the whole procedure takes about 2 hours. The procedure can be repeated as many times as you want, respecting a proper recovery interval. The total investment is around US$ 300, for 10 procedures. No air ticket, not hotel, etc.

I do believe it's worth a try, and I will in a close future.

Procedure:

1- Pluck the hair with a forceps or a good tweezer. 20 hair follicles are enough for 1cm2 and put them on a petri #1. Hair bulb have to come together (as intact as possible).

Petri#1

2- Add 10ml of 0.025% trypsin-EDTA solution to petri #1. Use a dropper.

http://www.lifetechnologies.com/order/catalog/product/25200056

US$ 11.85 (100ml)

3- Put petr #1 in a incubator for 30 min at 37 Celsius degree.

http://www.amazon.com/Xcellent-Global-Hatching-Incubator-F-HG034/dp/B00N780WCS/ref=sr_1_4?ie=UTF8&qid=1424486878&sr=8-4&keywords=7+egg+incubator

US$ 54.29

http://www.amazon.com/Unknown-5000-ReptiPro-6000-Incubator/dp/B007AG1M8Q/ref=sr_1_cc_4?s=aps&ie=UTF8&qid=1424488401&sr=1-4-catcorr&keywords=incubator+reptile

US$ 150.00

4- Transfer the hair follicles to another petri #2.

Petri#2

5- Add 5ml of trypsin inhibitor to petr #1 to avoid melanocyte digestion.

http://www.lifetechnologies.com/order/catalog/product/R007100

US$ 36.62 (100ml)

6- Keep petri #1 separated apart (waiting).

7- Add 10ml of 0.025% trypsin-EDTA to petri#2.

8- Put petr #2 in a incubator for 30 min.

9- Transfer the hair follicles to another petri #3.

Petri#3

10- Add 5ml of trypsin inhibitor to petr #2 to avoid melanocyte digestion.

11- Keep petri #2 separated apart (waiting).

12- Add 10ml of 0.025% trypsin-EDTA to petri#3

13- Put petr #3 in a incubator for 30 min. Add 5ml of trypsin inhibitor to petr #3 to avoid melanocyte digestion.

14- Throw the hair away and mix all petri (#1, #2, and #3) contents.

15- Filter the combined solution with a 70um strainer.

http://www.ebay.com/itm/40UM-70UM-100UM-CELL-STRAINER-Lot-of-6-by-Biologix-/191085124407?pt=LH_DefaultDomain_0&hash=item2c7d8f9b37

US$ 23.00 (6pack)

16- Centrifuge the strained solution during 5min at 1000RPM, so that it will preciptate.

http://www.ebay.com/itm/HFS-Electric-Centrifuge-Lab-Medical-Practice-4000-rpm-20ml-/281301409469?pt=LH_DefaultDomain_0&hash=item417ededebd

US$ 79.99

17- Remove the supernatant liquid and keep only the pellets .

18- After removing the supernatant liquid, add a 5-10ml of saline solution (PBS PH 7.4 or DMEM/F-12) to the pellets and mix it.

www.amazon.com/Teknova-Solution-7-4-1000mL-Sterile/dp/B00O86LQNM/ref=sr_1_3?ie=UTF8&qid=1424486471&sr=8-3&keywords=PBS+PH+7.4

US$ 39.00

19- You've got an autologus stem melanocyte suspension. Keep in the refrigerator.

20- Roll dermaroller (0.5mm needles) on the patches (both vertically and horizontally) twice, where suspension will acommodate. Use a dropper.

http://www.amazon.com/Microneedle-Conditions-Stimulating-Production-Treatments/dp/B009653PQ6/ref=sr_1_5?ie=UTF8&qid=1424486531&sr=8-5&keywords=dermaroller

US$ 7.00

21-The cells should be secured with a silicone netting (Mepitel®, Mölnlycke AB, Mölnlycke, Sweden) extending about 1 cm onto the dry non-dermarolled surrounding skin.

http://www.amazon.com/Mepitel-Wound-Dressing-3x4-Each/dp/B003XCTCCE/ref=sr_1_3?ie=UTF8&qid=1424486580&sr=8-3&keywords=mepitel

US$ 14.00

21- Then two layers of saline moistened woven gauze compresses semipermeable Tegaderm™ film are applied and glued in place onto the surrounding skin with Mastisol® (Ferndale Laboratories, Inc, Ferndale, MI, USA). The glue ensures that it will be secured in place for the period needed.

http://www.amazon.com/Tegaderm-Transparent-Dressing-2-375-Picture/dp/B0009Q2OMW/ref=sr_1_2?ie=UTF8&qid=1424486685&sr=8-2&keywords=tegaderm

US$ 8.24

and

http://www.amazon.com/Mastisol-Medical-Liquid-Adhesive-Bottle/dp/B006OU8PNY/ref=sr_1_1?ie=UTF8&qid=1424487467&sr=8-1&keywords=mastisol

US$ 17.00

22- Keep the dermarolled regions as stopped as possible during a week and also don't water or moisten them for the same time.

23-Good Luck.

Disclaimer:

Replies

Manu February 21, 2015 at 11:35pm

Hi Friends,

I went through this procedure thrice and it is effective, this is an OPD based procedure will take max 2-3 hours, and it is very effective for localized small patches, wherein for larger patches procedure has to be repeated depending upon the size. I went through this procedure on my foot ankles of both the legs (both sides) and the results were quite good although the entire patch has not been recovered fully, a small area is still effected, will undergo once again with the procedure and me and my doctor is very hopeful that this time the entire region will get recovered. Will update you guys once its done.

Thanks FLAVIO for sharing the useful info.

Cheers!!
- Robot > Manu February 22, 2015 at 5:01pm
  
  Thanks Manu.
  
  How did the Doctor treat the recipient area? Dermabrasion or dermastamp?
  
  Regards.
  - Manu > Robot July 4, 2015 at 8:18am
    
    Dermabrasion !
  - Bamsegutt > Robot March 8, 2015 at 5:02am
    
    Hey Flavio...I miss all this ...Good work.D
sukrutha February 21, 2015 at 10:03pm

OMG...Flavio...U are amazing, could you please share your experience with us....how are results after this treatment.
Ainara Iturbe Zumeta February 21, 2015 at 2:09am

Good job Flavio!

I see that you are working a lot with this item and that is appreciated. It's great :-)

Thank you for all you are doing!

Good Luck too!

:-)
- Robot > Ainara Iturbe Zumeta February 21, 2015 at 3:29am
  
  Thanks Ainara.
  
  I need replies from Friends who have also spent sometime on this subject.
  - Ainara Iturbe Zumeta > Robot February 21, 2015 at 10:49am
    
    And how can we help you?
    
    I don't know who are you steel, but I hope and think that you have good intentions.
    
    So if I could do something you only have to say me.
    - Robot > Ainara Iturbe Zumeta February 21, 2015 at 2:51pm
      
      I would like to cross information with people that has done any melanocyte transplantation.
      
      This procedure is not my invention. It's only a compilation of those books that you also had access.
      
      Also the following website: http://skinrephair.com/
      - Ainara Iturbe Zumeta > Robot February 21, 2015 at 9:47pm
        
        I read the website you send us, and at first it sounds good.
        
        On the other hand, I never have done any melanocyte transplantation, so with this item I couldn't help you, but you can count on me when you need it.

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